一种同时进行牛囊胚差异染色和凋亡染色的方法

doi:10.11843/j.issn.0366-6964.2015.11.008

Abstract

Abstract:

To evaluate the quality of bovine blastocysts efficiently and accurately，a triple immunofluorescence staining method was established in bovine blastocyst.The trophectoderm(TE) cells and apoptotic cells were identified with labeling of CDX2 protein and caspase-3 protein，respectively.All of nuclei in blastocyst cells were stained with Hochest 33342.Triple staining was designed as 3 schemes.PI was used to stain nuclei of TE cells and apoptotic cells were detected by TUNEL method in double staining.The results showed that the triple immunofluorescence staining，with 3 schemes，could distinguish the TE and apoptotic cells accurately.Additionally，co-incubation of 2 first antibodys/second antibodys was the most optimal method to triple staining in terms of accuracy，efficiency and stain duration.Therefore，in same blastocyst，identification the TE and apoptotic cells by labeling CDX2 and caspase-3 protein respectively was available to evaluate the quality of bovine blastocysts.Also，there were great advantages in triple staining compared with double staining.

CLC Number:

S823
S813

SUN Wei-jun，YU Xue-ying，HU Ting-xi，GUO Qin-qin，LIU Yan，HAO Hai-sheng，ZHAO Xue-ming，ZHU Hua-bin，DU Wei-hua. A Method Used for Differential Staining and Apoptosis Cell Staining in Bovine Blastocyst[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 1967-1973.

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A Method Used for Differential Staining and Apoptosis Cell Staining in Bovine Blastocyst

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